Antigen

Antigen Class

TFs and others

Antigen

ZNF654

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

dev stage

embryonic

donor ID

ENCDO000ABF

age

unknown

sex

female

lab

Michael Snyder, Stanford

health state

unknown

sample type

immortalized cell line

cell line

HEK293

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

general protocol: https://www.encodeproject.org/documents/0994a79b-44ae-49ef-a44c-6b2ebf85a230/@@download/attachment/ChIP-Seq%20Biorupter%20Pico%20BravoTruSeq%20protocol%20for%20Syapse%20%28modified%20for%20HEK293%20GFP-ZNF%29.pdf

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

26064402

Reads aligned (%)

91.1

Duplicates removed (%)

8.1

Number of peaks

21729 (qval < 1E-05)

hg19

Number of total reads

26064402

Reads aligned (%)

90.6

Duplicates removed (%)

8.2

Number of peaks

21324 (qval < 1E-05)